{"id":119,"date":"2020-07-22T12:15:06","date_gmt":"2020-07-22T09:15:06","guid":{"rendered":"https:\/\/systems.mbg.duth.gr\/?page_id=119"},"modified":"2021-11-26T13:20:05","modified_gmt":"2021-11-26T10:20:05","slug":"research","status":"publish","type":"page","link":"https:\/\/systems.mbg.duth.gr\/index.php\/research\/","title":{"rendered":"\u0388\u03c1\u03b5\u03c5\u03bd\u03b1"},"content":{"rendered":"<p><span style=\"font-family: helvetica, arial, sans-serif;\"><strong>\u0399. \u0391\u03bd\u03ac\u03c0\u03c4\u03c5\u03be\u03b7 \u03c4\u03b5\u03c7\u03bd\u03b9\u03ba\u03ce\u03bd \u03b3\u03b9\u03b1 \u03c4\u03b7\u03bd \u03bc\u03b5\u03bb\u03ad\u03c4\u03b7\u03c2 \u03c4\u03b7\u03c2 \u03b1\u03c1\u03c7\u03b9\u03c4\u03b5\u03ba\u03c4\u03bf\u03bd\u03b9\u03ba\u03ae\u03c2 \u03c4\u03b7\u03c2 \u03c7\u03c1\u03c9\u03bc\u03b1\u03c4\u03af\u03bd\u03b7\u03c2<\/strong><\/span><\/p>\n<div id=\"attachment_133\" style=\"width: 255px\" class=\"wp-caption alignright\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-133\" class=\"wp-image-133\" src=\"https:\/\/systems.mbg.duth.gr\/wp-content\/uploads\/2020\/07\/Fig3.eps_-300x274.png\" alt=\"\" width=\"245\" height=\"224\" srcset=\"https:\/\/systems.mbg.duth.gr\/wp-content\/uploads\/2020\/07\/Fig3.eps_-300x274.png 300w, https:\/\/systems.mbg.duth.gr\/wp-content\/uploads\/2020\/07\/Fig3.eps_-1024x934.png 1024w, https:\/\/systems.mbg.duth.gr\/wp-content\/uploads\/2020\/07\/Fig3.eps_-768x701.png 768w, https:\/\/systems.mbg.duth.gr\/wp-content\/uploads\/2020\/07\/Fig3.eps_-600x547.png 600w, https:\/\/systems.mbg.duth.gr\/wp-content\/uploads\/2020\/07\/Fig3.eps_.png 1220w\" sizes=\"auto, (max-width: 245px) 100vw, 245px\" \/><p id=\"caption-attachment-133\" class=\"wp-caption-text\"><span style=\"font-family: helvetica, arial, sans-serif; font-size: 8pt;\">Example of T2C (adapted and modified from Kolovos et al., 2018)<\/span><\/p><\/div>\n<p><span style=\"font-family: helvetica, arial, sans-serif;\">\u0391\u03bd\u03ac\u03c0\u03c4\u03c5\u03be\u03b7 \u03c4\u03b5\u03c7\u03bd\u03bf\u03bb\u03bf\u03b3\u03b9\u03ce\u03bd \u03c0\u03bf\u03c5 \u03b5\u03c0\u03b9\u03c4\u03c1\u03ad\u03c0\u03bf\u03c5\u03bd \u03c4\u03b7\u03bd \u03bc\u03b5\u03bb\u03ad\u03c4\u03b7 \u03c4\u03b7\u03c2 \u03c7\u03c9\u03c1\u03bf\u03c7\u03c1\u03bf\u03bd\u03b9\u03ba\u03ae\u03c2 \u03bf\u03c1\u03b3\u03ac\u03bd\u03c9\u03c3\u03b7\u03c2 \u03ba\u03b1\u03b9 \u03b1\u03c1\u03c7\u03b9\u03c4\u03b5\u03ba\u03c4\u03bf\u03bd\u03b9\u03ba\u03ae\u03c2 \u03c4\u03bf\u03c5 \u03b3\u03bf\u03bd\u03b9\u03b4\u03b9\u03ce\u03bc\u03b1\u03c4\u03bf\u03c2 \u03c3\u03b5 \u03b4\u03b9\u03ac\u03c6\u03bf\u03c1\u03b1 \u03b2\u03b9\u03bf\u03bb\u03bf\u03b3\u03b9\u03ba\u03ac \u03c3\u03c5\u03c3\u03c4\u03ae\u03bc\u03b1\u03c4\u03b1. \u03a4\u03b5\u03c7\u03bd\u03b9\u03ba\u03ad\u03c2 \u03cc\u03c0\u03c9\u03c2 \u03b7 3C-seq (<a href=\"https:\/\/www.nature.com\/articles\/nprot.2013.018?page=3\" target=\"_blank\" rel=\"noopener noreferrer\">Stadhouders, <em>et al<\/em>., 2013<\/a>) \u03ba\u03b1\u03b9 \u03c4\u03bf T2C (<a href=\"https:\/\/epigeneticsandchromatin.biomedcentral.com\/articles\/10.1186\/1756-8935-7-10\" target=\"_blank\" rel=\"noopener noreferrer\">Kolovos <em>et al<\/em>., 2014<\/a>; <a href=\"https:\/\/www.nature.com\/articles\/nprot.2017.132?proof=trueIn%25EF%25BB%25BF\" target=\"_blank\" rel=\"noopener noreferrer\">Kolovos <em>et al<\/em>., 2018<\/a>), \u03b5\u03c0\u03b9\u03c4\u03c1\u03ad\u03c0\u03bf\u03c5\u03bd \u03c4\u03b7\u03bd \u03b1\u03bd\u03ac\u03bb\u03c5\u03c3\u03b7 \u03c4\u03b7\u03c2 \u03b1\u03c1\u03c7\u03b9\u03c4\u03b5\u03ba\u03c4\u03bf\u03bd\u03b9\u03ba\u03ae\u03c2 \u03c4\u03b7\u03c2 \u03c7\u03c1\u03c9\u03bc\u03b1\u03c4\u03af\u03bd\u03b7\u03c2<\/span><\/p>\n<p><span style=\"font-family: helvetica, arial, sans-serif;\">\u03a3\u03c5\u03b3\u03ba\u03c1\u03b5\u03ba\u03c1\u03b9\u03bc\u03ad\u03bd\u03b1, \u03b7 \u03bc\u03ad\u03b8\u03bf\u03b4\u03bf\u03c2 T2C (<a href=\"https:\/\/epigeneticsandchromatin.biomedcentral.com\/articles\/10.1186\/1756-8935-7-10\" target=\"_blank\" rel=\"noopener noreferrer\">Kolovos <em>et al<\/em>., 2014<\/a>; <a href=\"https:\/\/www.nature.com\/articles\/nprot.2017.132?proof=trueIn%25EF%25BB%25BF\" target=\"_blank\" rel=\"noopener noreferrer\">Kolovos <em>et al<\/em>., 2018<\/a>) \u03b5\u03c0\u03b9\u03c4\u03c1\u03ad\u03c0\u03b5\u03b9 \u03c4\u03b7\u03bd \u03b1\u03bd\u03ac\u03bb\u03c5\u03c3\u03b7 \u03c4\u03b7\u03c2 \u03bf\u03c1\u03b3\u03ac\u03bd\u03c9\u03c3\u03b7\u03c2 \u03c4\u03b7\u03c2 \u03c7\u03c1\u03c9\u03bc\u03b1\u03c4\u03af\u03bd\u03b7\u03c2 \u03c3\u03c4\u03bf \u03c7\u03ce\u03c1\u03bf \u03ba\u03b1\u03b9 \u03c4\u03bf\u03bd \u03c7\u03c1\u03cc\u03bd\u03bf, \u03c4\u03b7\u03c2 \u03b4\u03b9\u03b1\u03bc\u03b5\u03c1\u03b9\u03c3\u03bc\u03b1\u03c4\u03bf\u03c0\u03bf\u03af\u03b7\u03c3\u03b7\u03c2, \u03ba\u03b1\u03b8\u03ce\u03c2 \u03ba\u03b1\u03b9 \u03c4\u03bf \u03b4\u03af\u03ba\u03c4\u03c5\u03bf \u03b3\u03bf\u03bd\u03b9\u03b4\u03b9\u03b1\u03ba\u03ce\u03bd \u03b1\u03bb\u03bb\u03b7\u03bb\u03b5\u03c0\u03b9\u03b4\u03c1\u03ac\u03c3\u03b5\u03c9\u03bd \u03c3\u03b5 \u03b9\u03b4\u03b9\u03b1\u03af\u03c4\u03b5\u03c1\u03b1 \u03c5\u03c8\u03b7\u03bb\u03ae \u03b1\u03bd\u03ac\u03bb\u03c5\u03c3\u03b7 (0.4 kbp) \u03ba\u03b1\u03b9 \u03ba\u03ac\u03bb\u03c5\u03c8\u03b7 \u03bc\u03b5 \u03b1\u03c1\u03ba\u03b5\u03c4\u03ac \u03c7\u03b1\u03bc\u03b7\u03bb\u03cc \u03ba\u03cc\u03c3\u03c4\u03bf\u03c2.<\/span><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><strong><span style=\"font-family: helvetica, arial, sans-serif;\">\u0399I. \u0397 \u03b1\u03c1\u03c7\u03b9\u03c4\u03b5\u03ba\u03c4\u03bf\u03bd\u03b9\u03ba\u03ae \u03c4\u03b7\u03c2 \u03c7\u03c1\u03c9\u03bc\u03b1\u03c4\u03af\u03bd\u03b7\u03c2 \u03ba\u03b1\u03b9 \u03bf \u03b4\u03b9\u03c4\u03c4\u03cc\u03c2 \u03c1\u03cc\u03bb\u03bf\u03c2 \u03c4\u03c9\u03bd \u03bc\u03b5\u03c4\u03b1\u03b3\u03c1\u03b1\u03c6\u03b9\u03ba\u03ce\u03bd \u03c0\u03b1\u03c1\u03b1\u03b3\u03cc\u03bd\u03c4\u03c9\u03bd<\/span><\/strong><\/p>\n<div id=\"attachment_143\" style=\"width: 257px\" class=\"wp-caption alignright\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-143\" class=\"wp-image-143\" src=\"https:\/\/systems.mbg.duth.gr\/wp-content\/uploads\/2020\/07\/Fig2-300x237.png\" alt=\"\" width=\"247\" height=\"195\" srcset=\"https:\/\/systems.mbg.duth.gr\/wp-content\/uploads\/2020\/07\/Fig2-300x237.png 300w, https:\/\/systems.mbg.duth.gr\/wp-content\/uploads\/2020\/07\/Fig2-1024x810.png 1024w, https:\/\/systems.mbg.duth.gr\/wp-content\/uploads\/2020\/07\/Fig2-768x608.png 768w, https:\/\/systems.mbg.duth.gr\/wp-content\/uploads\/2020\/07\/Fig2-600x475.png 600w, https:\/\/systems.mbg.duth.gr\/wp-content\/uploads\/2020\/07\/Fig2.png 1259w\" sizes=\"auto, (max-width: 247px) 100vw, 247px\" \/><p id=\"caption-attachment-143\" class=\"wp-caption-text\"><span style=\"font-family: helvetica, arial, sans-serif; font-size: 8pt;\">(Adapted and modified from Kolovos et al., 2016)<\/span><\/p><\/div>\n<p><span style=\"font-family: helvetica, arial, sans-serif;\">\u0397 \u03bc\u03b5\u03bb\u03ad\u03c4\u03b7 \u03c4\u03b7\u03c2 \u03b4\u03c5\u03bd\u03b1\u03bc\u03b9\u03ba\u03ae\u03c2 \u03c4\u03bf\u03c5 \u03c1\u03cc\u03bb\u03bf\u03c5 \u03ba\u03b1\u03b9 \u03c4\u03b7\u03c2 \u03b1\u03c0\u03cc\u03ba\u03c1\u03b9\u03c3\u03b7\u03c2 \u03c4\u03c9\u03bd \u03bc\u03b5\u03c4\u03b1\u03b3\u03c1\u03b1\u03c6\u03b9\u03ba\u03ce\u03bd \u03c0\u03b1\u03c1\u03b1\u03b3\u03cc\u03bd\u03c4\u03c9\u03bd \u03ba\u03b1\u03b9 \u03c4\u03b7\u03c2 \u03b1\u03c1\u03c7\u03b9\u03c4\u03b5\u03ba\u03c4\u03bf\u03bd\u03b9\u03ba\u03ae\u03c2 \u03c4\u03bf\u03c5 \u03b3\u03bf\u03bd\u03b9\u03b4\u03b9\u03ce\u03bc\u03b1\u03c4\u03bf\u03c2 \u03c3\u03b5 \u03b2\u03b9\u03bf\u03bb\u03bf\u03b3\u03b9\u03ba\u03ac \u03c3\u03c5\u03c3\u03c4\u03ae\u03bc\u03b1\u03c4\u03b1 \u03cc\u03c0\u03c9\u03c2 \u03b7 \u03b4\u03b9\u03b1\u03c6\u03bf\u03c1\u03bf\u03c0\u03bf\u03af\u03b7\u03c3\u03b7 \u03c4\u03bf\u03c5 \u03b1\u03b9\u03bc\u03bf\u03c0\u03bf\u03b9\u03b7\u03c4\u03b9\u03ba\u03bf\u03cd \u03c3\u03c5\u03c3\u03c4\u03ae\u03bc\u03b1\u03c4\u03bf\u03c2 (Kolovos <em>et al<\/em>., <em>in preparation<\/em>) \u03ba\u03b1\u03b9 \u03b7 \u03c6\u03bb\u03b5\u03b3\u03bc\u03bf\u03bd\u03ce\u03b4\u03b7\u03c2 \u03b1\u03c0\u03cc\u03ba\u03c1\u03b9\u03c3\u03b7 \u03b5\u03bd\u03b4\u03bf\u03b8\u03b7\u03bb\u03b9\u03b1\u03ba\u03ce\u03bd \u03ba\u03c5\u03c4\u03c4\u03ac\u03c1\u03c9\u03bd (<a href=\"https:\/\/genomebiology.biomedcentral.com\/articles\/10.1186\/s13059-014-0536-6\" target=\"_blank\" rel=\"noopener noreferrer\">Diermeier, <em>et al<\/em>. 2014<\/a>; <a href=\"https:\/\/genome.cshlp.org\/content\/26\/11\/1478.full\" target=\"_blank\" rel=\"noopener noreferrer\">Kolovos <em>et al<\/em>., 2016<\/a>). \u0391\u03c5\u03c4\u03ad\u03c2 \u03bf\u03b9 \u03bc\u03b5\u03bb\u03ad\u03c4\u03b5\u03c2 \u03b1\u03c0\u03bf\u03ba\u03ac\u03bb\u03c5\u03c8\u03b1\u03bd \u03ad\u03bd\u03b1 \u03c3\u03c5\u03bd\u03b4\u03c5\u03b1\u03c3\u03c4\u03b9\u03ba\u03cc \u03b4\u03af\u03ba\u03c4\u03c5\u03bf \u03bc\u03b5\u03c4\u03b1\u03b3\u03c1\u03b1\u03c6\u03b9\u03ba\u03ce\u03bd \u03c0\u03b1\u03c1\u03b1\u03b3\u03cc\u03bd\u03c4\u03c9\u03bd \u03c0\u03bf\u03c5 \u03b5\u03bb\u03ad\u03b3\u03c7\u03bf\u03c5\u03bd \u03c4\u03b7\u03bd \u03b3\u03bf\u03bd\u03b9\u03b4\u03b9\u03b1\u03ba\u03ae \u03ad\u03ba\u03c6\u03c1\u03b1\u03c3\u03b7 \u03bc\u03ad\u03c3\u03c9 \u03c0\u03c1\u03bf\u03b4\u03b9\u03b1\u03b3\u03b5\u03b3\u03c1\u03b1\u03bc\u03bc\u03ad\u03bd\u03c9\u03bd \u03b1\u03bb\u03bb\u03b7\u03bb\u03b5\u03c0\u03b9\u03b4\u03c1\u03ac\u03c3\u03b5\u03c9\u03bd \u03c4\u03b7\u03c2 \u03c7\u03c1\u03c9\u03bc\u03b1\u03c4\u03af\u03bd\u03b7\u03c2 (prelooping).<\/span><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-family: helvetica, arial, sans-serif;\"><strong>\u0399I\u0399. \u0397 \u03b3\u03bf\u03bd\u03b9\u03b4\u03b9\u03b1\u03ba\u03ae \u03c1\u03cd\u03b8\u03bc\u03b9\u03c3\u03b7 \u03ba\u03b1\u03b9 \u03ad\u03ba\u03c6\u03c1\u03b1\u03c3\u03b7 \u03c9\u03c2 \u03b1\u03c0\u03cc\u03ba\u03c1\u03b9\u03c3\u03b7 <\/strong><\/span><strong>\u03c3\u03b5 \u03c3\u03b7\u03bc\u03b1\u03c4\u03bf\u03b4\u03bf\u03c4\u03b9\u03ba\u03ac \u03bc\u03bf\u03bd\u03bf\u03c0\u03ac\u03c4\u03b9\u03b1<\/strong><\/p>\n<div id=\"attachment_486\" style=\"width: 220px\" class=\"wp-caption alignright\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-486\" class=\"wp-image-486 \" src=\"https:\/\/systems.mbg.duth.gr\/wp-content\/uploads\/2020\/10\/Picture1-e1603266555628-300x278.png\" alt=\"\" width=\"210\" height=\"195\" srcset=\"https:\/\/systems.mbg.duth.gr\/wp-content\/uploads\/2020\/10\/Picture1-e1603266555628-300x278.png 300w, https:\/\/systems.mbg.duth.gr\/wp-content\/uploads\/2020\/10\/Picture1-e1603266555628.png 543w\" sizes=\"auto, (max-width: 210px) 100vw, 210px\" \/><p id=\"caption-attachment-486\" class=\"wp-caption-text\"><span style=\"font-family: helvetica, arial, sans-serif; font-size: 8pt;\">\u039c\u03bf\u03bd\u03c4\u03ad\u03bb\u03bf \u03b3\u03b9\u03b1 \u03c4\u03bf\u03bd \u03b4\u03b9\u03c4\u03c4\u03cc \u03c1\u03cc\u03bb\u03bf \u03c4\u03bf\u03c5 NF\u03ba\u0392 \u03c3\u03c4\u03b7 \u03c6\u03bb\u03b5\u03b3\u03bc\u03bf\u03bd\u03ce\u03b4\u03b7 \u03b1\u03c0\u03cc\u03ba\u03c1\u03b9\u03c3\u03b7, \u03c3\u03b5 HUVECs, (results concern Kolovos et al., 2016))<\/span><\/p><\/div>\n<p style=\"text-align: left;\"><span style=\"font-family: helvetica, arial, sans-serif;\">\u0397 \u03bc\u03b5\u03bb\u03ad\u03c4\u03b7 \u03ba\u03b1\u03b9 \u03b7 \u03b1\u03bd\u03ac\u03bb\u03c5\u03c3\u03b7 \u03c4\u03b7\u03c2 \u03b4\u03c5\u03bd\u03b1\u03bc\u03b9\u03ba\u03ae\u03c2 \u03c4\u03c9\u03bd \u03b5\u03bd\u03b9\u03c3\u03c7\u03c5\u03c4\u03ce\u03bd\/\u03c5\u03c0\u03bf\u03ba\u03b9\u03bd\u03b7\u03c4\u03ce\u03bd (poised\/active\/silenced) \u03ba\u03b1\u03b9 \u03c4\u03b7\u03c2 \u03bf\u03c1\u03b3\u03ac\u03bd\u03c9\u03c3\u03b7\u03c2 \u03c4\u03b7\u03c2 3D \u03b1\u03c1\u03c7\u03b9\u03c4\u03b5\u03ba\u03c4\u03bf\u03bd\u03b9\u03ba\u03ae\u03c2 \u03c4\u03bf\u03c5 \u03b3\u03bf\u03bd\u03b9\u03b4\u03b9\u03ce\u03bc\u03b1\u03c4\u03bf\u03c2 \u03c3\u03c4\u03b1 \u03c0\u03bb\u03b1\u03af\u03c3\u03b9\u03b1 \u03c4\u03c9\u03bd \u03c3\u03b7\u03bc\u03b1\u03c4\u03bf\u03b4\u03bf\u03c4\u03b9\u03ba\u03ce\u03bd \u03bc\u03bf\u03bd\u03bf\u03c0\u03b1\u03c4\u03b9\u03ce\u03bd TNF\u03b1 \u03ba\u03b1\u03b9 TGF, \u03bc\u03ad\u03c3\u03c9 <\/span><span style=\"font-family: helvetica, arial, sans-serif;\">\u03b4\u03b9\u03b1\u03c6\u03cc\u03c1\u03c9\u03bd \u03bc\u03b5\u03c4\u03b1\u03b3\u03c1\u03b1\u03c6\u03b9\u03ba\u03ce\u03bd \u03c0\u03b1\u03c1\u03b1\u03b3\u03cc\u03bd\u03c4\u03c9\u03bd \u03b5\u03bd\u03b5\u03c1\u03b3\u03ce\u03bd \u03c3\u03b5 \u03b1\u03c5\u03c4\u03ac \u03c4\u03b1 \u03bc\u03bf\u03bd\u03bf\u03c0\u03ac\u03c4\u03b9\u03b1 \u03ba\u03b1\u03b9 \u03b7 \u03b5\u03c0\u03af\u03b4\u03c1\u03b1\u03c3\u03ae \u03c4\u03bf\u03c5\u03c2 \u03c3\u03c4\u03b7\u03bd \u03b3\u03bf\u03bd\u03b9\u03b4\u03b9\u03b1\u03ba\u03ae \u03c1\u03cd\u03b8\u03bc\u03b9\u03c3\u03b7 \u03ba\u03b1\u03b9 \u03ad\u03ba\u03c6\u03c1\u03b1\u03c3\u03b7.<\/span><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u0399V. \u0395\u03c0\u03b9\u03b3\u03b5\u03bd\u03b5\u03c4\u03b9\u03ba\u03ae \u03ba\u03b1\u03b9 \u03c3\u03c7\u03ad\u03c3\u03b7 \u03c4\u03b7\u03c2 \u03bc\u03b5 \u03c4\u03b7\u03bd \u03c1\u03cd\u03b8\u03bc\u03b9\u03c3\u03b7 \u03c4\u03b7\u03c2 \u03b3\u03bf\u03bd\u03b9\u03b4\u03b9\u03b1\u03ba\u03ae\u03c2 \u03ad\u03ba\u03c6<\/strong><strong>\u03c1\u03b1\u03c3\u03b7\u03c2<\/strong><\/p>\n<div id=\"attachment_448\" style=\"width: 310px\" class=\"wp-caption alignright\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-448\" class=\"wp-image-448 size-medium\" src=\"https:\/\/systems.mbg.duth.gr\/wp-content\/uploads\/2020\/08\/Kolovos_Nishimura_Sankar_et_al_Fig5.v2-300x151.png\" alt=\"\" width=\"300\" height=\"151\" srcset=\"https:\/\/systems.mbg.duth.gr\/wp-content\/uploads\/2020\/08\/Kolovos_Nishimura_Sankar_et_al_Fig5.v2-300x151.png 300w, https:\/\/systems.mbg.duth.gr\/wp-content\/uploads\/2020\/08\/Kolovos_Nishimura_Sankar_et_al_Fig5.v2-1024x515.png 1024w, https:\/\/systems.mbg.duth.gr\/wp-content\/uploads\/2020\/08\/Kolovos_Nishimura_Sankar_et_al_Fig5.v2-768x386.png 768w, https:\/\/systems.mbg.duth.gr\/wp-content\/uploads\/2020\/08\/Kolovos_Nishimura_Sankar_et_al_Fig5.v2-1536x772.png 1536w, https:\/\/systems.mbg.duth.gr\/wp-content\/uploads\/2020\/08\/Kolovos_Nishimura_Sankar_et_al_Fig5.v2-600x301.png 600w, https:\/\/systems.mbg.duth.gr\/wp-content\/uploads\/2020\/08\/Kolovos_Nishimura_Sankar_et_al_Fig5.v2.png 1608w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><p id=\"caption-attachment-448\" class=\"wp-caption-text\"><span style=\"font-family: helvetica, arial, sans-serif; font-size: 8pt;\">(Adapted and modified from Kolovos et al., 2020)<\/span><\/p><\/div>\n<p><span style=\"font-family: helvetica, arial, sans-serif;\">\u039f\u03b9\u00a0<span class=\"st\">\u03c0\u03c1\u03c9\u03c4\u03b5\u0390\u03bd\u03b5\u03c2 polycomb (PRC1\/2) \u03b5\u03af\u03bd\u03b1\u03b9 \u03c3\u03b7\u03bc\u03b1\u03bd\u03c4\u03b9\u03ba\u03ad\u03c2 \u03b3\u03b9\u03b1 \u03c4\u03b7\u03bd \u03c1\u03cd\u03b8\u03bc\u03b9\u03c3\u03b7 \u03c4\u03b7\u03c2 <\/span><span class=\"st\">\u03b3\u03bf\u03bd\u03b9\u03b4\u03b9\u03b1\u03ba\u03ae\u03c2 \u03ad\u03ba\u03c6\u03c1\u03b1\u03c3\u03b7\u03c2. \u039c\u03b5\u03bb\u03b5\u03c4\u03bf\u03cd\u03bc\u03b5 \u03c4\u03b7\u03bd \u03c3\u03c7\u03ad\u03c3\u03b7 \u03c4\u03bf\u03c5 \u03c3\u03c5\u03bc\u03c0\u03bb\u03cc\u03ba\u03bf\u03c5 Polycomb <\/span><span class=\"st\">repressive de-<span style=\"font-family: helvetica, arial, sans-serif;\">ubiquitinase (PR-DUB) \u03bc\u03b5 \u03c4\u03b9\u03c2 PRC1\/2 \u03ba\u03b1\u03b9 \u03c4\u03bf\u03c5 \u03c1\u03cc\u03bb\u03bf\u03c5 \u03c4\u03bf\u03c5 \u03c3\u03c4\u03b7\u03bd \u03b4\u03b9\u03b1\u03c4\u03ae\u03c1\u03b7\u03c3\u03b7 \u03c4\u03b7\u03c2 \u03c7\u03c1\u03c9\u03bc\u03b1\u03c4\u03af\u03bd\u03b7\u03c2 \u03c3\u03b5 \u03bc\u03b9\u03b1 \u03b9\u03b4\u03b1\u03bd\u03b9\u03ba\u03ae \u03ba\u03b1\u03c4\u03ac\u03c3\u03c4\u03b1\u03c3\u03b7, \u03bc\u03ad\u03c3\u03c9 \u03b5\u03c0\u03b9\u03b3\u03b5\u03bd\u03b5\u03c4\u03b9\u03ba\u03ce\u03bd \u03bc\u03b7\u03c7\u03b1\u03bd\u03b9\u03c3\u03bc\u03ce\u03bd, \u03ce\u03c3\u03c4\u03b5 \u03bd\u03b1 \u03b5\u03bb\u03ad\u03b3\u03c7\u03b5\u03b9 \u03ba\u03b1\u03b9 \u03bd\u03b1 \u03c1\u03c5\u03b8\u03bc\u03af\u03b6\u03b5\u03b9 \u03c4\u03b7\u03bd \u03ad\u03ba\u03c6\u03c1\u03b1\u03c3\u03b7 <\/span><\/span><span class=\"st\">\u03b3\u03bf\u03bd\u03b9\u03b4\u03af\u03c9\u03bd <\/span><span class=\"st\">\u03c3\u03b7\u03bc\u03b1\u03bd\u03c4\u03b9\u03ba\u03ce\u03bd \u03b3\u03b9\u03b1 \u03b4\u03b9\u03ac\u03c6\u03bf\u03c1\u03b5\u03c2 \u03bb\u03b5\u03b9\u03c4\u03bf\u03c5\u03c1\u03b3\u03af\u03b5\u03c2 \u03c4\u03bf\u03c5 \u03bf\u03c1\u03b3\u03b1\u03bd\u03b9\u03c3\u03bc\u03bf\u03cd (<a href=\"https:\/\/genome.cshlp.org\/content\/30\/8\/1119.full\" target=\"_blank\" rel=\"noopener noreferrer\">Kolovos <em>et al<\/em>., 2020<\/a><\/span><span class=\"st\">).<\/span><\/span><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-family: helvetica, arial, sans-serif;\"><strong><strong>V. \u03a4\u03b1\u03c5\u03c4\u03bf\u03c0\u03bf\u03af\u03b7\u03c3\u03b7 \u03ba\u03b1\u03b9\u03bd\u03bf\u03cd\u03c1\u03b9\u03c9\u03bd \u03bc\u03b5\u03c4\u03b1\u03b3\u03c1\u03b1\u03c6\u03b9\u03ba\u03ce\u03bd \u03c0\u03b1\u03c1\u03b1\u03b3\u03cc\u03bd\u03c4\u03c9\u03bd<\/strong><\/strong><\/span><\/p>\n<p><span style=\"font-family: helvetica, arial, sans-serif;\">H \u03bc\u03b5\u03bb\u03ad\u03c4\u03b7 \u03bc\u03b5 \u03bc\u03b5\u03b8\u03cc\u03b4\u03bf\u03c5\u03c2 screening (CRISPR-Cas9) \u03b3\u03b9\u03b1 \u03c4\u03b7\u03bd \u03c4\u03b1\u03c5\u03c4\u03bf\u03c0\u03bf\u03af\u03b7\u03c3\u03b7 \u03ba\u03b1\u03b9\u03bd\u03bf\u03cd\u03c1\u03b9\u03c9\u03bd \u03bc\u03b5\u03c4\u03b1\u03b3\u03c1\u03b1\u03c6\u03b9\u03ba\u03ce\u03bd \u03c0\u03b1\u03c1\u03b1\u03b3\u03cc\u03bd\u03c4\u03c9\u03bd \u03ba\u03b1\u03b9 \u03c4\u03bf\u03c5 \u03c1\u03cc\u03bb\u03bf\u03c5 \u03c4\u03bf\u03c5\u03c2 \u03c3\u03c4\u03b7 \u03b4\u03b7\u03bc\u03b9\u03bf\u03c5\u03c1\u03b3\u03af\u03b1 \u03ba\u03b1\u03b9 \u03b4\u03b9\u03b1\u03c6\u03bf\u03c1\u03bf\u03c0\u03bf\u03af\u03b7\u03c3\u03b7 \u03c4\u03c9\u03bd \u03b1\u03b9\u03bc\u03bf\u03c0\u03bf\u03b9\u03b7\u03c4\u03b9\u03ba\u03ce\u03bd \u03b2\u03bb\u03b1\u03c3\u03c4\u03b9\u03ba\u03ce\u03bd \u03ba\u03c5\u03c4\u03c4\u03ac\u03c1\u03c9\u03bd (HSCs) \u03bc\u03ad\u03c3\u03c9 \u03c4\u03b7\u03c2 \u03c4\u03c1\u03b9\u03c3\u03b4\u03b9\u03ac\u03c3\u03c4\u03b1\u03c4\u03b7\u03c2 \u03b1\u03c1\u03c7\u03b9\u03c4\u03b5\u03ba\u03c4\u03bf\u03bd\u03b9\u03ba\u03ae\u03c2 \u03c4\u03bf\u03c5 \u03b3\u03bf\u03bd\u03b9\u03b4\u03b9\u03ce\u03bc\u03b1\u03c4\u03bf\u03c2. \u039f \u03c3\u03ba\u03bf\u03c0\u03cc\u03c2 \u03b5\u03af\u03bd\u03b1\u03b9 \u03b7 \u03b1\u03c0\u03bf\u03ba\u03b1\u03bb\u03cd\u03c8\u03b7 \u03c4\u03bf\u03c5 \u03c4\u03c1\u03cc\u03c0\u03bf\u03c5 \u03b4\u03b9\u03b1\u03c6\u03bf\u03c1\u03bf\u03c0\u03bf\u03af\u03b7\u03c3\u03b7\u03c2 \u03c4\u03bf\u03c5 \u03b1\u03b9\u03bc\u03bf\u03c0\u03bf\u03b9\u03b7\u03c4\u03b9\u03ba\u03bf\u03cd \u03c3\u03c5\u03c3\u03c4\u03ae\u03bc\u03b1\u03c4\u03bf\u03c2 \u03ba\u03b1\u03b9 \u03b7 \u03c0\u03b1\u03c1\u03b1\u03b3\u03c9\u03b3\u03ae \u03c4\u03c9\u03bd \u03b4\u03b9\u03b1\u03c6\u03bf\u03c1\u03b5\u03c4\u03b9\u03ba\u03ce\u03bd \u03c4\u03cd\u03c0\u03c9\u03bd \u03ba\u03c5\u03c4\u03c4\u03ac\u03c1\u03c9\u03bd, \u03ce\u03c3\u03c4\u03b5 \u03bd\u03b1 \u03ba\u03b1\u03c4\u03b1\u03bd\u03bf\u03ae\u03c3\u03bf\u03c5\u03bc\u03b5 \u03c4\u03bf \u03bc\u03bf\u03c1\u03b9\u03b1\u03ba\u03cc \u03c5\u03c0\u03bf\u03b2\u03ac\u03b8\u03c1\u03bf \u03c4\u03b7\u03c2 \u03b1\u03b9\u03bc\u03bf\u03c0\u03bf\u03af\u03b7\u03c3\u03b7\u03c2.<br \/>\n<\/span><\/p>\n<p>&nbsp;<\/p>\n<div id=\"attachment_668\" style=\"width: 257px\" class=\"wp-caption alignright\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-668\" class=\"wp-image-668\" src=\"https:\/\/systems.mbg.duth.gr\/wp-content\/uploads\/2021\/11\/Screenshot-2021-11-26-at-10.07.23-AM-300x284.png\" alt=\"\" width=\"247\" height=\"234\" srcset=\"https:\/\/systems.mbg.duth.gr\/wp-content\/uploads\/2021\/11\/Screenshot-2021-11-26-at-10.07.23-AM-300x284.png 300w, https:\/\/systems.mbg.duth.gr\/wp-content\/uploads\/2021\/11\/Screenshot-2021-11-26-at-10.07.23-AM.png 703w\" sizes=\"auto, (max-width: 247px) 100vw, 247px\" \/><p id=\"caption-attachment-668\" class=\"wp-caption-text\"><span style=\"font-family: helvetica, arial, sans-serif; font-size: 8pt;\">Circular genome map of L. plantarum L125 (Adapted from Tegopoulos et al., 2021)<\/span><\/p><\/div>\n<p><strong><span style=\"font-family: helvetica, arial, sans-serif;\">VI. \u0391\u03bd\u03ac\u03bb\u03c5\u03c3\u03b7 \u03bc\u03b9\u03ba\u03c1\u03bf\u03b2\u03b9\u03ce\u03bc\u03b1\u03c4\u03bf\u03c2 \u03ba\u03b1\u03b9 \u03b3\u03bf\u03bd\u03b9\u03b4\u03b9\u03ce\u03bc\u03b1\u03c4\u03bf\u03c2 \u03b2\u03b1\u03ba\u03c4\u03b7\u03c1\u03af\u03c9\u03bd<img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-666\" src=\"https:\/\/systems.mbg.duth.gr\/wp-content\/uploads\/2021\/11\/Figure1.tif\" alt=\"\" width=\"1\" height=\"1\" \/><\/span><\/strong><\/p>\n<p><span style=\"font-family: helvetica, arial, sans-serif;\">\u0391\u03bd\u03ac\u03c0\u03c4\u03c5\u03be\u03b7 \u03bc\u03b5\u03b8\u03bf\u03b4\u03bf\u03bb\u03bf\u03b3\u03b9\u03ce\u03bd \u03b1\u03bd\u03ac\u03bb\u03c5\u03c3\u03b7\u03c2 \u03bc\u03b9\u03ba\u03c1\u03bf\u03b2\u03b9\u03ce\u03bc\u03b1\u03c4\u03bf\u03c2 \u03ba\u03b1\u03b9 \u03b3\u03bf\u03bd\u03b9\u03b4\u03b9\u03ce\u03bc\u03b1\u03c4\u03bf\u03c2 \u03b2\u03b1\u03ba\u03c4\u03b7\u03c1\u03af\u03c9\u03bd, \u03bc\u03b5 \u03c3\u03ba\u03bf\u03c0\u03cc \u03c4\u03b7\u03bd <em>in silico<\/em>\u00a0\u03c4\u03b1\u03be\u03b9\u03bd\u03bf\u03bc\u03b9\u03ba\u03ae \u03ba\u03b1\u03b9 \u03c6\u03c5\u03bb\u03bf\u03b3\u03b5\u03bd\u03b5\u03c4\u03b9\u03ba\u03ae \u03c4\u03bf\u03c0\u03bf\u03b8\u03ad\u03c4\u03b7\u03c3\u03ae \u03c4\u03bf\u03c5\u03c2, \u03ba\u03b1\u03b8\u03ce\u03c2 \u03ba\u03b1\u03b9 \u03c4\u03b7\u03bd <em>in silico<\/em>\u00a0\u03b1\u03bd\u03ac\u03bb\u03c5\u03c3\u03b7 \u03c4\u03bf\u03c5 \u03b3\u03bf\u03bd\u03b9\u03b4\u03b9\u03ce\u03bc\u03b1\u03c4\u03bf\u03c2 \u03c4\u03bf\u03c5\u03c2 \u03bc\u03b5 \u03b1\u03c0\u03bf\u03c4\u03ad\u03bb\u03b5\u03c3\u03bc\u03b1 \u03c4\u03b7\u03bd \u03c0\u03c1\u03cc\u03b2\u03bb\u03b5\u03c8\u03b7 \u03c4\u03c9\u03bd \u03b4\u03b9\u03b1\u03c6\u03bf\u03c1\u03b5\u03c4\u03b9\u03ba\u03ce\u03bd \u03c0\u03b9\u03b8\u03b1\u03bd\u03ce\u03bd \u03b9\u03b4\u03b9\u03bf\u03c4\u03ae\u03c4\u03c9\u03bd \u03c4\u03c9\u03bd \u03b2\u03b1\u03ba\u03c4\u03b7\u03c1\u03af\u03c9\u03bd (\u03c0\u03c7 \u03c0\u03c1\u03bf\u03b2\u03b9\u03bf\u03c4\u03b9\u03ba\u03ae, \u03b8\u03b5\u03c1\u03b1\u03c0\u03b5\u03c5\u03c4\u03b9\u03ba\u03ae, \u03ba\u03c4\u03bb.) \u03ba\u03b1\u03b9 \u03c3\u03c4\u03b7\u03bd \u03c3\u03c5\u03bd\u03ad\u03c7\u03b5\u03b9\u03b1 \u03c4\u03b7\u03bd \u03b5\u03c5\u03ba\u03bf\u03bb\u03cc\u03c4\u03b5\u03c1\u03b7 \u03ba\u03b1\u03b9 \u03c3\u03c4\u03bf\u03c7\u03b5\u03c5\u03bc\u03ad\u03bd\u03b7 <em>in vitro<\/em> \u03b5\u03c0\u03b9\u03b2\u03b5\u03b2\u03b1\u03af\u03c9\u03c3\u03b7 \u03c4\u03bf\u03c5\u03c2 (<a href=\"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2021.746659\/full\">Stergiou <em>et al<\/em>., 2021<\/a>, <a href=\"https:\/\/www.mdpi.com\/2227-9059\/9\/11\/1718\/htm\">Tegopoulos <em>et al<\/em>., 2021<\/a>).<\/span><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u0399. \u0391\u03bd\u03ac\u03c0\u03c4\u03c5\u03be\u03b7 \u03c4\u03b5\u03c7\u03bd\u03b9\u03ba\u03ce\u03bd \u03b3\u03b9\u03b1 \u03c4\u03b7\u03bd \u03bc\u03b5\u03bb\u03ad\u03c4\u03b7\u03c2 \u03c4\u03b7\u03c2 \u03b1\u03c1\u03c7\u03b9\u03c4\u03b5\u03ba\u03c4\u03bf\u03bd\u03b9\u03ba\u03ae\u03c2 \u03c4\u03b7\u03c2 \u03c7\u03c1\u03c9\u03bc\u03b1\u03c4\u03af\u03bd\u03b7\u03c2 \u0391\u03bd\u03ac\u03c0\u03c4\u03c5\u03be\u03b7 \u03c4\u03b5\u03c7\u03bd\u03bf\u03bb\u03bf\u03b3\u03b9\u03ce\u03bd \u03c0\u03bf\u03c5 \u03b5\u03c0\u03b9\u03c4\u03c1\u03ad\u03c0\u03bf\u03c5\u03bd \u03c4\u03b7\u03bd \u03bc\u03b5\u03bb\u03ad\u03c4\u03b7 \u03c4\u03b7\u03c2 \u03c7\u03c9\u03c1\u03bf\u03c7\u03c1\u03bf\u03bd\u03b9\u03ba\u03ae\u03c2 \u03bf\u03c1\u03b3\u03ac\u03bd\u03c9\u03c3\u03b7\u03c2 \u03ba\u03b1\u03b9 \u03b1\u03c1\u03c7\u03b9\u03c4\u03b5\u03ba\u03c4\u03bf\u03bd\u03b9\u03ba\u03ae\u03c2 \u03c4\u03bf\u03c5 \u03b3\u03bf\u03bd\u03b9\u03b4\u03b9\u03ce\u03bc\u03b1\u03c4\u03bf\u03c2 \u03c3\u03b5 \u03b4\u03b9\u03ac\u03c6\u03bf\u03c1\u03b1 \u03b2\u03b9\u03bf\u03bb\u03bf\u03b3\u03b9\u03ba\u03ac \u03c3\u03c5\u03c3\u03c4\u03ae\u03bc\u03b1\u03c4\u03b1. \u03a4\u03b5\u03c7\u03bd\u03b9\u03ba\u03ad\u03c2 \u03cc\u03c0\u03c9\u03c2 \u03b7 3C-seq (Stadhouders, et al., 2013) \u03ba\u03b1\u03b9 \u03c4\u03bf T2C (Kolovos et al., 2014; Kolovos et al., 2018), \u03b5\u03c0\u03b9\u03c4\u03c1\u03ad\u03c0\u03bf\u03c5\u03bd \u03c4\u03b7\u03bd \u03b1\u03bd\u03ac\u03bb\u03c5\u03c3\u03b7 \u03c4\u03b7\u03c2 \u03b1\u03c1\u03c7\u03b9\u03c4\u03b5\u03ba\u03c4\u03bf\u03bd\u03b9\u03ba\u03ae\u03c2 \u03c4\u03b7\u03c2 \u03c7\u03c1\u03c9\u03bc\u03b1\u03c4\u03af\u03bd\u03b7\u03c2 \u03a3\u03c5\u03b3\u03ba\u03c1\u03b5\u03ba\u03c1\u03b9\u03bc\u03ad\u03bd\u03b1, \u03b7&hellip; <br \/> <a class=\"read-more\" href=\"https:\/\/systems.mbg.duth.gr\/index.php\/research\/\">Read more<\/a><\/p>\n","protected":false},"author":16,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-119","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/systems.mbg.duth.gr\/index.php\/wp-json\/wp\/v2\/pages\/119","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/systems.mbg.duth.gr\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/systems.mbg.duth.gr\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/systems.mbg.duth.gr\/index.php\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/systems.mbg.duth.gr\/index.php\/wp-json\/wp\/v2\/comments?post=119"}],"version-history":[{"count":145,"href":"https:\/\/systems.mbg.duth.gr\/index.php\/wp-json\/wp\/v2\/pages\/119\/revisions"}],"predecessor-version":[{"id":680,"href":"https:\/\/systems.mbg.duth.gr\/index.php\/wp-json\/wp\/v2\/pages\/119\/revisions\/680"}],"wp:attachment":[{"href":"https:\/\/systems.mbg.duth.gr\/index.php\/wp-json\/wp\/v2\/media?parent=119"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}